Pierce™ Monomeric Avidin Agarose Kit, Thermo Scientific

Supplier: LIFE TECHNOLOGIES MS

20227
PI20227EA 711.38 USD
PI20227
Pierce™ Monomeric Avidin Agarose Kit, Thermo Scientific
Protein Purification Protein Purification Kits

The Thermo Scientific Pierce™ Monomeric Avidin Kit enables mild affinity purification of biotinylated proteins, peptides and other molecules


  • Monomeric Avidin Agarose can be regenerated and reused at least 10 times
  • Retains biotin-binding specificity and low nonspecific binding of native avidin
  • Bind samples in a variety of physiological buffers
  • Binding capacity >1.2 mg biotinylated BSA/mL resin


During monomeric avidin immobilization, polymeric forms of avidin with strong binding characteristics also are immobilized. These high affinity biotin-binding sites must first be blocked with a biotin-containing buffer. Biotin molecules are eluted from monomers with a glycine solution revealing only the reversible binding sites. The biotinylated molecule of interest may then be applied to the support for purification and then eluted by ligand competition using a biotin-containing solution.


Avidin monomers have a much lower biotin-binding affinity than native tetrameric avidin enabling dissociation of biotinylated molecules using mild elution conditions


Información de suministro: Kit includes Pierce™ Monomeric Avidin column, 2 mL (1 column), PBS pack, makes 500 mL (1 pack), Biotin Blocking and Elution Buffer (200 mL), Regeneration Buffer (250 mlL, and column accessories.


Precaución: For Research Use Only. Not for use in diagnostic procedures.

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