Pierce™ RNA 3' End Desthiobiotinylation Kit, Thermo Scientific

Supplier: LIFE TECHNOLOGIES MS

20163
PI20163EA 900 USD
PI20163
Pierce™ RNA 3' End Desthiobiotinylation Kit, Thermo Scientific
Protein Linking and Labeling Systems

The Thermo Scientific Pierce RNA 3'-End Desthiobiotinylation Kit contains reagents for easy and efficient desthiobiotin labeling of RNA for use as probes or as antibody alternatives for enrichment of RNA binding proteins.


  • Non-radioactive— a single biotin labels has similar detection sensitivity as radioactivity
  • End-labeled—results in minimal disturbance of RNA secondary structure
  • Flexible—desthiobiotin label may be used for detection or as an affinity handle for streptavidin resin


The Pierce RNA 3'-End Desthiobiotinylation Kit is optimized for labeling the 3'-end of single-stranded RNA using T4 RNA ligase. Once labeled, the RNA can be used as a probe or target for gel-shift EMSA reactions, northern blots and protein-RNA interaction experiments. The desthiobiotinylated RNA also can be used to enrich for RNA binding proteins (RBP) using streptavidin affinity resin.


Información de suministro: Kit includes desthiobiotinylated cytidine bisphosphate (40 µL), T4 RNA ligase (40 µL), T4 RNA ligase reaction buffer,10X (100 µL), Non-labeled RNA control (100 µL), Biotinylated IRE RNA control (35 µL), RNase inhibitor (2x10 µL), DMSO (200 µL), PEG 30% (300 µL), Glycogen (20 µL) and Nuclease-free water (1,5 mL).


Precaución: For Research Use Only. Not for use in diagnostic procedures.

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