Chymotrypsin, Promega

Supplier: Promega Corporation

V1062
PAV1062EA 348.34 USD
PAV1062
Chymotrypsin, Promega
Enzymes

Chymotrypsin is a highly-purified serine endopeptidase derived from bovine pancreas that preferentially hydrolyzes at the carboxyl side of aromatic amino acids: Tyr, Phe and Trp. Cleavage may also be observed, but at a lower rate, at Leu and Met.


  • Serine Protease that Cleaves at C-Terminus of Tyr, Phe and Trp
  • Optimal activity at pH 7.0-9.0.
  • Use alone or with other proteases for peptide mapping, peptide mass fingerprinting, MS/MS spectral matching
  • Use for digestion reactions in-solution or in-gel


Chymotrypsin is a highly-purified serine endopeptidase derived from bovine pancreas that preferentially hydrolyzes at the carboxyl side of aromatic amino acids: Tyr, Phe and Trp. Cleavage may also be observed, but at a lower rate, at Leu and Met. Chymotrypsin activity is optimal in the pH range of 7.0-9.0. This sequencing grade enzyme can be used alone or in combination with other proteases to produce protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.


It is suitable for in-solution or in-gel digestion reactions.


Chymotrypsin activity is optimal in the pH range of 7.0–9.0. This sequencing-grade enzyme can be used alone or in combination with other proteases to produce protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching.

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