CSM-His-Leu-Lys-Trp-Ura (Powder), MP Biomedicals

Supplier: MP BIOMEDICALS (FKA ICN BIOMED

114550022
IC114550022EA 837.01 USD
IC114550022
CSM-His-Leu-Lys-Trp-Ura (Powder), MP Biomedicals
Cell Culture Media

Five dropout complete supplement mixture used to select for yeast containing plasmids with specific nutritional markers and to select for gene function in transformation experiments.


  • Used for S. cerevisiae growth media
  • Soluble in water (2 g/l)


Add 0.55 g of CSM-His-Leu-Lys-Trp-Ura to each liter of DOB or DOBA and autoclave at 121 °C for 25 minutes for complete synthetic defined single drop-out medium for S.cerevisiae.


Five dropout of complete supplement mixture (CSM) of amino acids for S. cerevisiae growth media. Time tested formulation is available in complete and dropout formulations.


Reagents are tested and chosen for their ability to promote optimum growth of yeast strains when combined in the DOB or DOBA with CSM-His-Leu-Ura formula. DOB (drop out base) is 1.7 g yeast nitrogen base, 5 g ammonium sulfate, 20 g dextrose per liter. DOBA is DOB with 17% agar. Chosen reagents are combined in the proper proportions according to the formulation listed in the certificate of analysis and milled to a powder which thoroughly blends the reagents for uniform distribution. A sample of the milled and blended formulation was used to prepare liquid plate medium by adding 43.7 g DOBA powder and 0.55 g of CSM-His-Leu-Lys-Trp-Ura to a liter of purified water. The combinations were mixed by hand for about 1 minute to dissolve the dextrose and autoclaved at 121 °C for 25 minutes. After cooling to 50 °C plates were poured.


Application includes: Growth of Saccharomyces cerevisiae,yeast cell culture, gene expression, transformation, gene function, gene regulation, aelection of yeast transformants, For confirmation of auxotrophic phenotypes.


Certificaciones: Molecular Biology Certified™.

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