Phosphodiesterase I, MP Biomedicals

Supplier: MP BIOMEDICALS (FKA ICN BIOMED

0210097880
IC10097880EA 410.06 USD
IC10097880
Phosphodiesterase I, MP Biomedicals
Enzymes

One unit hydrolyzes 1 µmole of p-nitrophenyl thymidine-5-phosphate per minute at pH 8.9 and 25 °C.


Activators: The enzyme has an absolute requirement for Mg2+; Philipps indicates an optimum concentration of 15 mM. Inhibitors: Reducing agents such as gluctathione, cysteine and ascorbic acid. It is completely inhibited by 5 mM EDTA while ATP, ADP and AMP are partial inhibitors. Composition: A glycoprotein which binds concanavalin A.


Phosphodiesterase I breaks phosphodiester bonds and catalyzes the hydrolysis of various nucleotide polyphosphates. Phosphodiesterase I is released from eucaryotic plasma membranes by phosphatidylinositol-specific phospholipase C.

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