E. coli Exonuclease III

Supplier: Biosearch Technologies

EX4425K
76085-632EA 496.19 USD
76085-632
E. coli Exonuclease III
Enzymes

Digest duplex DNA in a 3´ to 5´ direction from a nick, blunt end, or 3´-recessed end, producing stretches of ssDNA


  • Produce stretches of ssDNA in dsDNA templates containing nicks, blunt ends or recessed 3' ends
  • Protect protruding 3' ends becuase this exonuclease will not start digestion at protruding 3' overhangs


Exonuclease III digests duplex DNA in a 3´ to 5´ direction from a nick, a blunt end, or 3´-recessed end, producing stretches of ssDNA on the opposite strand. Under defined reaction conditions, DNA degradation by Exonuclease III proceeds at a uniform rate yielding predictable and reproducible digestion results.


Because the rate of exonucleolytic excision of deoxyribonucleotides by Exonuclease III is dependent upon reaction factors including temperature, ionic strength, template sequence, and enzyme-to-DNA ratios, each template must be optimized using sample digestions to achieve the desired excision rate.


Exonuclease III is not active on 3´-protruding ends of four bases or more in length, ssDNA, or on thioester-linked nucleotides. The enzyme also has intrinsic RNase H, 3´-DNA phosphatase, and apurinic DNA endonuclease activities.


Certificaciones: For research use only. Not for human or diagnostic use.


Información para pedidos: Contents include enzyme and a 10X reaction buffer.

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